Professor Bannister is one of the three secondary villains in animated television horror film My Little Pony: Rogue Murderer. He is voiced by Kevin McDonald, who also voiced Pleakley in Lilo and Stitch series (2002-2006).
Bannister, while passing through Ponyville with fellow bikers Bradley Buttowski (Danny Cooksey) and Suzy Johnson (Kari Wahlgren), stops at a convenience store, where he and his friends start harassing two customers Rarity (Tabitha St. Germain) and Pinkie Pie (Andrea Libman). Grabbing Pinkie with Bradley, Bannister holds her, while Suzy taunts Rarity, making Rarity grudgingly ask for Pinkie's earlier thrown wallet and call her "Ms. Johnson". When Suzy eventually does give Rarity the wallet, Bannister and Bradley let Pinkie go.
Shortly, after having their motorcycles wrecked by Pinkie as revenge for troubling her and Rarity, Bannister and his companions follow the two mares to Sweet Apple Acres, where Bannister, keeping a lookout signals for his friends to come out of hiding when the coast is clear. Along with Bradley, Bannister begins siphoning gas from Applejack's orange Volkswagen beetle, planning on using the fuel to set fire to the barn. When Bradley fills up a canister of gas, Bannister grabs it and heads to the barn, where he finds Suzy, who had earlier wandered off, happily swinging from a suspended rope and pulley on the second floor of the building. Demanding Suzy to come down and help him, Bannister fails to notice Fauntleroy Donaldson, grab her, take her inside, and kill her in cold blood off-screen. Going into the barn to look for Suzy, Bannister climbs up onto the second floor and finds Suzy dead, having been pinned to a barn rafter by her throat with a sharp pitchfork.
Staring in horror at Suzy's corpse, Bannister is attacked by Fauntleroy, who impales him by the stomach with another pitchfork. After killing Bannister in cold blood, Fauntleroy rolls his corpse onto the bottom floor of the barn, and later hangs him upside-down from an apple tree. Bannister's corpse, still suspended from the apple tree, is later found by the authorities and taken away by the paramedics. In the deleted scene, Twilight Sparkle (Tara Strong) found his bloody corpse tied to a chair in her library.
- In the original Phineas and Ferb novel, Professor Bannister is a minor villain that doesn't appears until page 223.